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O-serogroups, virulence genes of pathogenic Escherichia coli and Pulsed-field gel electrophoresis (PFGE) patterns of O149 isolates from diarrhoeic piglets in Korea
Escherichia coli piglet O-serogroup pathotype virulence gene
2015/4/16
A total of 116 Escherichia (E.) coli isolates isolated from neonatal diarrhoeic piglets were serogrouped and tested for the presence of virulence genes for fimbrial and non-fimbrial adhesins, intimin,...
Influence of the Escherichia coli Nissle 1917 strain on complications of the chronic experimental liver damage
bacterial translocation liver cirrhosis probiotics spontaneous bacterial peritonitis thioacetamide
2015/3/13
The aim of the study was evaluate the influence of the probiotic Escherichia coli Nissle 1917 strain (Mutaflor® suspension, Ardeypharm GmbH, Herdecke, Germany) on bacterial translocation in cases...
【目的】为了构建MDV1细菌人工染色体，以便快速有效地进行重组病毒的筛选，进行了含E.coli F因子的马立克氏病病毒1型（MDV1）转移载体的构建。【方法】利用DNA重组技术，构建了含有E.coli F因子的马立克氏病病毒1型（MDV1）转移载体，利用脂质体，将线性化的转移载体转染入已感染CVI988病毒的CEF，用MX-HAT药物筛选3代以后，用X-gal染色,挑取阳性克隆。【结果】经测序分析...