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Identification and Fine Mapping of a Gene Related to Pale Green Leaf Phenotype near the Centromere Region in Rice (Oryza sativa)
centromere gene fine mapping pale green leaf mutant chlorophyll a chlorophyll b
2008/12/11
A thermo-insensitive pale green leaf mutant (pgl2) was isolated from T-DNA inserted transgenic lines of rice (Oryza sativa L. subsp. japonica cv. Nipponbare). Genetic analysis indicated that the pheno...
Relationship Between the First Base of the Donor Splice Site of Waxy Gene Intron 1 and Amylose Content in Yunnan Indigenous Rice Varieties
Yunnan indigenous rice varieties waxy gene intron the first base of donor splice site
2008/12/11
There exists a single nucleotide polymorphism, G or T, at the first base of the donor splice site of waxy gene intron 1 in rice. In order to study the relationship between the first base of the donor ...
Expression and Characterization of a UDP-Glucose Pyrophosphorylase Gene in Cotton
Expression Characterization UDP-Glucose Pyrophosphorylase Gene Cotton
2008/12/9
UDP-glucose is the primary substrate for cellulose synthase, the enzyme that produces the main chemical constituent of cotton fiber. Two enzymes, UDP-glucose pyrophosphorylase (UDPGp, EC 2.7.7.9) and ...
SSR Marker(s) Associated with Root Knot Nematode Resistance Gene(s) in Cotton
Cotton Root Knot Nematode Resistance
2008/5/9
Root-knot nematode [Meloidogyne incognita (Kofoid and White) Chitwood] is a major pest on cotton (Gossypium hirsutum L.) across the U. S. Cotton Belt. DNA markers that enable markerassisted selection ...
Acquisition of Thermotolerance in Transgenic Orchardgrass Plants with DgHSP17.2 Gene
Orchardgrass (Dactylis glomerata L.) Agrobacterium-mediated Transformation DgHSP17.2 Gene Thermotolerance
2016/4/27
To develop transgenic orchardgrass (Dactylis glomerata L.) resistant to high temperature, the recombinant DgHSP17.2 gene was introduced into orchardgrass plants using the Agrobacterium-mediated transf...
Associations between Alu I Polymorphism in the Prolactin Receptor Gene and Reproductive Traits of Slovak Large White, White Meaty and Landrace Pigs
Prolactin Receptor Gene PRLR Reproductive Traits Litter Size Pigs
2016/4/27
We investigated the effect of the prolactin receptor gene (PRLR) on total number of born (TNB), number of born alive (NBA) and number of weaned (NW) piglets in Large White (LW), White Meaty (WM) and L...
野油菜黄单胞菌S-152遗传转移系统的建立及外源性内切葡聚糖酶基因在其体内的表达Development of Genetic transfer System and Expression of an Extraneous Endoglucanase gene in xanthomonas s-152
野油菜黄单胞菌 细胞接合 遗传转移系统 内切葡聚糖酶 基因表达
2008/1/20
摘要
(通过细胞接合的方式将广宿主质粒pRK404、pKT230和RP4分别转入野油菜黄单胞菌S-152中,其转移频率均在10-4数量级上,从而建立起了S-152的转移系统。用同法将由载体质粒pRK404和白纹黄单胞菌XA1-1的CMC酶基因构成的重组质粒pND82转入到S-152中并得到了表达,使S-152(pND82)的胞内CMC酶酶活比出发菌株S-152提高了1倍。The wide-hos...
应用寡核苷酸芯片分析水稻花序相关基因在花序发育中的表达谱Gene Expression in Rice Inflorescence Development was Monitoredby Using Oligonucleotide Microarray
寡核苷酸芯片 水稻花序相关基因 基因差异表达
2008/1/16
摘要从Internet、国内外文献中查询了50个水稻花序的相关基因,制备成水稻花序相关基因的寡核苷酸芯片。对3个不同生长阶段的水稻花序材料进行了表达谱检测,用ScanArray3000对杂交结果进行扫描,得到了不同的基因表达谱。用ImaGene 4.0软件对获得的表达谱进行分析,获得基因表达差异的散点图及饼图。 图像分析表明,候选基因在水稻花序3个不同发育阶段的材料中,表达水平有显著差异。这些结果...
家蚕核型多角体病毒山东株p35基因的克隆及序列分析Cloning and Sequencing of p35 Gene from Bombyx mori Nuclear Polyhedrosis Virus Strain Shandong
家蚕核型多角体病毒山东株 p35基因 克隆 序列分析
2008/1/16
摘要根据家蚕核型多角体病毒T3株p35基因非编码区序列设计一对特异性引物,应用PCR技术从家蚕核型多角体病毒山东株中扩增得到一条1 080 bp的片段。测序结果表明,该片段含有p35基因,开放阅读框长900 bp,编码299个氨基酸,分子量为34.91kDa,pI=6.4。序列分析表明,BmNPV山东株的p35基因与T3株存在差异。
Abstract:According to the 5′ an...
甘蓝型油菜Ogura 雄性不育×白菜的回交杂种后代与亲本之间蕾期基因表达差异比较研究*Differences of Gene Expression in Bud Stage of Backcross Hybrid Between Ogura-type Male-Sterile Brassica napus L and B campestris L versus Parents
Ogura雄性不育 白菜 蕾期 基因表达 cDNA-AFLP 远缘杂交 回交
2008/1/10
摘要
以甘蓝型油菜(Brassica napus L, AACC,2n=38)Ogura细胞质雄性不育材料为母本,以不同白菜(B campestris ssp. chinensis Makino, AA, 2n=20) 自交系‘新选一号’和‘矮脚黄’为父本进行杂交,获得了杂种F1、BC1、BC2代。利用cDNA-AFLP技术对两种材料的不同回交世代BC1、BC2代与其亲本在蕾期的基因表达进行分析...
马铃薯密码子用法分析及其在t-PA基因密码子改造上的应用Analysis of Codon Usage in Potato and Its Application in the Modification of t-PA Gene
马铃薯 t-PA 密码子用法 优越密码子 密码子改造
2008/1/10
摘要 采用bioperl-1.0 工具在红旗LINUX系统下自编了密码子分析软件;通过对马铃薯98个蛋白质编码基因序列(codon DNA sequence)的分析,计算出了马铃薯的密码子用法,并确定出了马铃薯的4个高表达优越密码子;依据马铃薯密码子用法和高表达优越密码子分析结果,对t-PA基因序列进行了密码子的改造,得到了具有马铃薯密码子使用特点的t-PA基因序列,从而为以马铃薯为生物反应器高效...
多浪羊MHC-DRB3基因座的PCR-RFLP多态性分析Polymorphism Analysis of MHC-DRB3 Gene in Dolang Sheep with PCR-RFLP
多浪羊 MHC-DRB3 分子遗传多态性 PCR-RFLP
2008/1/10
摘要 主要组织相容性复合体(MHC)是由紧密连锁的高度多态的基因位点所组成的染色体上的一个遗传区域,它在动物机体的免疫系统中发挥着非常重要的作用。应用PCR-RFLP技术首次对多浪羊的MHC-DRB3的外显子2进行分子遗传多态性检测与分析。结果显示,多浪羊MHC-DRB3基因的外显子2在TaqⅠ、PstⅠ和HaeⅢ酶切位点存在多态,其酶切位点分别由2、2和6种共显性等位基因控制。综合3种酶切结果,...
蒙古山羊和哈萨克山羊GOLA-DRB3基因的HaeⅢ酶切多态性分析Polymorphism Analysis of the GOLA-DRB3 Gene Digested with HaeⅢ in Mongolian Goat and Kazakh Goat
蒙古山羊 哈萨克山羊 GOLA-DRB3 PCR-RFLP
2008/1/10
摘要
采用限制性内切核酸酶HaeⅢ对蒙古山羊和哈萨克山羊GOLA-DRB3基因外显子2的285bp扩增产物进行了PCR-RFLP多态性分析,共检测到17种基因型,由A、B、C、D、E、F和H等7个复等位基因控制;通过酶切图谱分析发现蒙古山羊和哈萨克山羊的GOLA-DRB3基因外显子2的154、168和220位碱基表现出多态性。并对基因型频率和等位基因频率进行了统计分析,结果表明,GOLA-DR...
摘要
以绵羊BMP15基因和BMPR-IB基因为候选基因,以湖羊、中国美利奴单胎品系、中国美利奴肉用和毛用多胎品系为研究对象,采用PCR-RFLP方法对候选基因进行单核苷酸多态性(SNP)位点检测和基因型分析,同时研究基因对绵羊产羔数的影响。对BMP15基因进行SNP检测,结果未发现多态性位点;对BMPR-IB基因进行多态性检测,结果发现了一个A746 G SNP位点。依据A746 G SNP位...
应用多重PCR和基因扫描技术对五指山猪13个家系32个微卫星基因座的遗传分析Genetic Analysis of 32 Microsatellite Loci in 13 Families of Wuzhishan Pig by Multiplex PCR and Gene scaning Technique
五指山猪 微卫星 基因扫描 遗传多样性
2008/1/6
摘要
五指山猪是我国著名的珍稀畜种,体型小、性成熟早、遗传稳定、近交程度高,可用于医学、药学和生物材料等方面。作者利用多重PCR和基因扫描技术对五指山猪13个家系的32个微卫星基因座进行遗传检测。统计各家系的等位基因组成,计算各家系的平均杂合度和多态信息含量(PIC);结果,各家系32基因座的平均等位基因数为13.66个,平均PIC为0.731,平均杂合度为0.559。表明海南五指山猪具有丰富的...