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The distribution of genes with positive and negative effects were symmetrical to nearly symmetrical for 1000-seed weight, charcoal rot disease resistance, fruiting branches/plant, capsules/plant, sing...
摘要利用抗病基因保守序列筛选大豆cDNA文库,获得一抗病基因同源cDNA片段,命名为KR3-1。根据KR3-1设计两个基因特异引物(GSP 和 NGSP),分别与通用引物(UPM)和巢式通用引物(NUP)共同扩增,成功地克隆到了该基因的5′末端序列。该扩增片段长447 bp,与已知序列重叠部分为129 bp。 Abstract:Based on part of a known partial c...
摘要雄性不育的研究对于杂种优势的利用具有重要意义。本文综述了小麦雄性不育遗传及基因定位研究进展,介绍了小麦雄性不育的基因工程,对小麦雄性不育的应用进行了讨论。 Abstract:The study of plant male sterility plays an important role on utilization of heterosis.This paper reviews the c...
摘要选用常用的两种报告基因、两种抗性基因、两种启动子序列和两种终止子序列为探针,将其PCR扩增产物用MicroGrid Ⅱ型全自动点样仪按矩阵排列点样于包埋有氨基的载玻片上,制备成转基因作物检测型基因芯片。利用该芯片对4种转基因水稻、木瓜、大豆、玉米进行检测,结果表明,该芯片能对转基因作物做出快速、准确的检测。 Abstract:Some selected available sequence...
摘要采用cDNA-AFLP(cDNA-amplified fragment length polymorphism)技术,分析“一粒传”后代小麦耐盐突变体中,耐盐性不同的两种小麦品系盐胁迫前后的基因表达差异,得到大量与耐盐相关的基因片段。其中73号片段与人类转录因子基因有一定的同源性(相应部分的同源性32%),命名为SIR73(salt inducible in resistance)。North...
摘要用花粉管通道法将柞蚕抗菌肽D基因导入番茄,获得了转基因植株。应用PCR技术、DNA斑点杂交以及Southern印迹分析表明,抗菌肽D基因已整合到番茄基因组;而且,位于目的基因下游的胭脂碱合成酶基因有表达。青枯假单孢菌活体接种结合高发病大田种植试验结果显示,部分转基因植株的子一代具有较强的抗青枯病能力。 Abstract:By using pollen-tube pathway,antibac...
期刊信息 篇名 Disruption of Smad5 gene leads to menhanced proliferation of High-proliferative potential precursors during embryonic hematopoiesis 语种 英文 撰写或编译 撰写 作者 Bing Liu,Yanxun Sun,Feizi Jiang,Shuangxi Z...
期刊信息 篇名 Combination of homoeologous pairing gene phKL and Ph2- deficiency in common wheat and its meiotic behaviors in hybrids with alien species. 语种 英文 撰写或编译 撰写 作者 Liu Deng-cai,Zheng You-liang,Yan Ze...
We investigated the effect of ESR gene-based selection on an improvement of litter size in the herds in real (non-experimental) conditions. The pigs were selected for three years. In the tested popula...
The objective of this study was to develop a simplified, efficient, and accurate protocol for sexing goat embryos. Based on the amelogenin gene located on the conservation region of X- and Y- chromoso...
Testis-specific protein (TSPY) is a Y-specific gene, with up to 200 copy numbers in bulls. In order to make bovine embryo sexing under farm condition more feasible, the possibility of using a non-elec...
Leptin is the adipocyte-specific product of the obese gene and plays a major role in food intake and energy metabolism. Leptin research was mainly focused on mammalian species, but understanding of le...
Use of RAPD and SCAR markers for identification of strawberry genotypes carrying red stele (Phytophtora fragariae) resistance gene Rpf1.
Thirty-six hybrids generated from crossing three lines with twelve testers were studied along with parents for combining ability and gene action in basmati rice. The general combining ability (GCA) an...
The objectives of this study were to confirm a location of the calpastatin (CAST) gene in chromosome 2 and to detect associations of genetic variations with economic traits in the porcine CAST gene as...

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